Artificial light and sun compass orientation in the sandhopper Talitrus saltator (Crustacea, Amphipoda)

Experiments on compass orientation under artificial light were conducted with adult individuals of Talitrus saltator. The aim was to reproduce in the laboratory an orientation based on the sun compass corresponding to that recorded in conditions of the true sun and sky. This was obtained by the creation within an opaque Plexiglas dome of a scenario that permitted variation of the brightness of the artificial sky and sun. The results show that it is possible to obtain sun compass orientation corresponding to the natural situation even in an artificial environment. It can be concluded that sandhoppers identify an artificial light source as the sun if the artificial sky is also illuminated and if the intensities of the artificial sun and sky exceed certain threshold values (1.13 and 10 μW cm⁻², respectively). The results of other experiments under the natural blue sky with an artificial sun and with the real sun attenuated are discussed. Accepted: 23 May 1997     

Integration of multi-objective structural optimization into cementless hip prosthesis design: Improved Austin-Moore model

A new strategy of multi-objective structural optimization is integrated into Austin-Moore prosthesis in order to improve its performance. The new resulting model is so-called Improved Austin-Moore. The topology optimization is considered as a conceptual design stage to sketch several kinds of hollow stems according to the daily loading cases. The shape optimization presents the detailed design stage considering several objectives. Here, A new multiplicative formulation is proposed as a performance scale in order to define the best compromise between several requirements. Numerical applications on 2D and 3D problems are carried out to show the advantages of the proposed model.     

A structured decision making analysis to increase a Red-cockaded Woodpecker population and balance stakeholder objectives for a National Forest

Longleaf pine (Pinus palustris) savanna is a biodiverse ecosystem native to the southeastern United States. Due to fire suppression and timber harvest, longleaf pine has declined to 3% of its original range. The extant forest provides habitat for many threatened and endangered species, including the endangered Red-cockaded Woodpecker (Picoides borealis; RCW), a cavity-nesting bird that co-evolved with fire-maintained forest, resulting in savanna-like old-growth forest as the bird’s preferred habitat. Our study site, the Oakmulgee Ranger District of the Talladega National Forest, harbors the largest RCW population in Alabama and is managed with an emphasis on RCW conservation. The United States Forest Service (USFS) also manages the Oakmulgee for uses such as wildlife conservation, recreation, and timber harvest. Despite efforts to restore RCW habitat and install artificial cavities in the Oakmulgee, the number of RCW groups has not exceeded 123, although the Recovery Plan objective is 394 groups. Our project was motivated by the USFS expressing interest in determining why the RCW population has not exceeded 123 groups. We proposed using structured decision making (SDM) with the USFS and other stakeholders to address this problem. Our goals were to explicitly define management objectives, build a Bayesian belief network with a model of how decision alternatives are believed to affect management objectives, and use a sensitivity analysis to determine the part of the model to which RCW population growth was most sensitive. Therefore, results from the analysis were expected to give insights into 1) ecological factors limiting RCW population growth, 2) how management can overcome these limits, plus 3) the relative expected ability of different decision alternatives to satisfy multiple objectives in addition to increasing RCW group number. We held four SDM workshops with representatives from the USFS, the Animal and Plant Health Inspection Service, the Longleaf Alliance, the Birmingham Audubon Society, and local residents. Stakeholder objectives consisted of maximizing the following: RCW group number, forest health, recreational enjoyment, community economic health, and aesthetics. Cavity insert installation had the greatest probability of increasing the number of RCW groups. The number of RCW groups was most affected by cavity availability, adult survival, reproductive output, food availability, and herbaceous understory. Prescribed burning was most likely to meet the combination of stakeholder objectives, followed by midstory removal. Our findings suggest that cavity installation efforts may need to be increased in the Oakmulgee to increase RCW group number. Also it could be beneficial to investigate how RCWs select cavity tree locations with the goal of increasing the chance that RCWs use artificial cavities to form new groups. The Bayesian belief network provided insights into factors limiting RCW population growth and how management can overcome these limits. The Bayesian belief network also can be used to prioritize management methods in the Oakmulgee given stakeholder objectives and time constraints.     

Do soft drinks affect metal ions release from orthodontic appliances?

ObjectiveThe effect of orange juice and Coca Cola^® on the release of metal ions from fixed orthodontic appliances.Materials and methodsA continuous flow system designed for in vitro testing of orthodontic appliances was used. Orange juice/Coca Cola^® was flowing through the system alternately with artificial saliva for 5.5 and 18.5 h, respectively. The collected samples underwent a multielemental ICP-OES analysis in order to determine the metal ions release pattern in time.ResultsThe total mass of ions released from the appliance into orange juice and Coca Cola^® (respectively) during the experiment was calculated (μg): Ni (15.33; 37.75), Cr (3.604; 1.052), Fe (48.42; ≥156.1), Cu (57.87, 32.91), Mn (9.164; 41.16), Mo (9.999; 30.12), and Cd (0.5967; 2.173).ConclusionsIt was found that orange juice did not intensify the release of metal ions from orthodontic appliances, whereas Coca Cola^® caused increased release of Ni ions.     

Artificial cultivation of true morels: current state,issues and perspectives

Morels (Morchella, Ascomycota), which are some of the most highly prized edible and medicinal mushrooms, are of great economic and scientific value. Morel cultivation has been a research focus worldwide for more than 100 years, and the outdoor cultivation of morels has succeeded and expanded to a large scale in China in recent years. In this study, we review the progress in recent research regarding the life cycle and reproductive systems in the genus Morchella and the current state of outdoor cultivation. Sclerotia formation and conidia production are two important phases during the life cycle. The morel species cultivated commercially in America is M. rufobrunnea based on molecular phylogenetic analysis. The species currently cultivated in China are black morels, including M. importuna, M. sextalata and M. eximia. The field cultivation of morels expanded in the majority of the provinces in China with a yield of fresh morels of 0–7620?kg per ha. The key techniques include spawn production, land preparation and spawning, the addition of exogenous nutrition, fruiting management and harvesting. The application of exogenous nutrition is the most important breakthrough in the field of morel cultivation, but the mechanism remains unclear. It was estimated that the total amount of field cultivated fresh morels was ～500 t in 2015–2016. We also discuss the potential issues remaining in the current literature and suggest directions for future studies.     

Water current velocity as an environmental factor regulating the distribution of amphipod species in Gamo lagoon, Japan

The role of current velocity as an environmental factor affecting the distribution of amphipod species was investigated in the brackish Gamo lagoon, Japan. Two gammaridean amphipod species, Eogammarus possjeticus and Melita setiflagella, were found inhabiting stations of different water current with same substrates. E. possjeticus and M. setiflagella selected substrates at low and high water current, respectively. The lifestyles and anatomical features of these two free-living amphipods contributed to their adaptation to habitats with different water currents. The results of field and experimental observations suggest the existence of some type of interspecific competition between the two species. The presence of E. possjeticus caused a displacement of the fundamental niche of M. setiflagella. Received: October 10, 2000 / Accepted: August 17, 2001     

A Conservative Point Mutation in a Dynamic Antigen-binding Loop of Human Immunoglobulin λ6 Light Chain Promotes Pathologic Amyloid Formation

Immunoglobulin light chain (LC) amyloidosis (AL) is a life-threatening human disease wherein free mono-clonal LCs deposit in vital organs. To determine what makes some LCs amyloidogenic, we explored patient-based amyloidogenic and non-amyloidogenic recombinant LCs from the λ6 subtype prevalent in AL. Hydrogen-deuterium exchange mass spectrometry, structural stability, proteolysis, and amyloid growth studies revealed that the antigen-binding CDR1 loop is the least protected part in the variable domain of λ6 LC, particularly in the AL variant. N32T substitution in CRD1 is identified as a driver of amyloid formation. Substitution N32T increased the amyloidogenic propensity of CDR1 loop, decreased its protection in the native structure, and accelerated amyloid growth in the context of other AL substitutions. The destabilizing effects of N32T propagated across the molecule increasing its dynamics in regions ∼30 Å away from the substitution site. Such striking long-range effects of a conservative point substitution in a dynamic surface loop may be relevant to Ig function. Comparison of patient-derived and engineered proteins showed that N32T interactions with other substitution sites must contribute to amyloidosis. The results suggest that CDR1 is critical in amyloid formation by other λ6 LCs.     

LATITUDINAL VARIATION OF WING:THORAX SIZE RATIO AND WING-ASPECT RATIO IN DROSOPHILA MELANOGASTER

In dipterans, the wing-beat frequency, and, hence, the lift generated, increases linearly with ambient temperature. If flight performance is an important target of natural selection, higher wing:thorax size ratio and wing-aspect ratio should be favored at low temperatures because they increase the lift for a given body weight. We investigated this hypothesis by examining wing: thorax size ratio and wing-aspect ratio in Drosophila melanogaster collected from wild populations along a latitudinal gradient and in their descendants reared under standard laboratory conditions. In a subset of lines, we also studied the phenotypic plasticity of these traits in response to temperature. To examine whether the latitudinal trends in wing:thorax size ratio and wing-aspect ratio could have resulted from a correlated response to latitudinal selection on wing area, we investigated the correlated responses of these characters in lines artificially selected for wing area. In both the geographic and the artificially selected lines, wing:thorax size ratio and wing-aspect ratio decreased in response to increasing temperature during development. Phenotypic plasticity for either trait did not vary among latitudinal lines or selective regimes. Wing:thorax size ratio and wing-aspect ratio increased significantly with latitude in field-collected flies. The cline in wing:thorax size ratio had a genetic component, but the cline in wing-aspect ratio did not. Artificial selection for increased wing area led to a statistically insignificant correlated increase in wing:thorax size ratio and a decrease in wing-aspect ratio. Our observations are consistent with the hypotheses that high wing-thorax size ratio and wing aspect ratio are per se selectively advantageous at low temperatures.     

Potential and peril of field experimentation: the use of copper to manipulate molluscan herbivores

Barriers of copper-based anti-fouling paints have become a popular method for the experimental manipulation of certain molluscan grazers, presumably by acting as a chemical barrier to movement. Although probably similar in action, copper metal can be more versatile in its application and maintenance. Its efficacy was confirmed in field experiments which examined the colonization of artificial surfaces by the intertidal red alga Halosaccion glandiforme (Rupr.). However, even though copper has the desired indirect effect on algae (i.e., the exclusion of grazers), it can still have undesirable direct effects as well, and these “costs of manipulation” must be recognized. These results demonstrate the necessity of proper controls in field experimentation, a consideration absent in many past studies. The general use of partial manipulations as control treatments appears useless for estimating artifacts associated with the use of copper barriers in particular and for other experimental manipulations in general. More meaningful controls may rely on finding multiple ways of manipulating the variable of interest.